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KMID : 0381120080300040355
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Genes and Genomics
2008 Volume.30 No. 4 p.355 ~ p.363
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hnRNP E1 Interacts with hnRNP L
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Choi Mie-Young
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Abstract
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Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is one of the principal pre-mRNA binding proteins found in human cells. The hnRNP L protein is a nucleocytoplasmic shuttling protein. It is composed of 558 amino acid residues and harbors four loosely-conserved RNP-consensus RNA-binding domains. In an attempt to identify the binding partners of hnRNP L, yeast two-hybrid screening was conducted using a human liver cDNA library. One of the cDNA clones was determined to harbor a partial human hnRNP E1 (poly(rC) binding protein 1) cDNA (GeneBank accession number XM_031585). In this study, I have determined that hnRNP L interacts specifically with the hnRNP E1 in the yeast two-hybrid system. This interaction was verified via in vitro pull-down assay.
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KEYWORD
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heterogeneous nuclear ribonucleoproteins (hnRNPs), RNP-concensus RNA-binding domain, hnRNP K homology domain (KH domain), yeast two-hybrid screening system, hnRNP E1 (poly(rC) binding protein 1)
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